Bioenno is currently marketing the DAB Substrate Kit (Catalog Number: 003836).  DAB (3,3’-diaminobenzidine) is a chromogen popularly used in immunohistochemistry, immunocytochemistry, and immunoblotting. The Bioenno DAB Substrate Kit is ideal for immuno-labeling on tissue sections and cells to detect the activity of peroxidase (HRP). The brown DAB-HRP reaction product is alcohol and heat resistant, and is also suitable for electron microscopy (EM). The kit contains all of the necessary reagents to prepare about 330 ml of substrate working solution, and the stock solutions are contained in convenient dropper bottles. The kit can be stored in a dark area at 2-8ºC and is stable for 12 months.

Production Description and Protocol

• DAB Substrate Kit Protocol

Bioenno Tech has successfully used the DAB Substrate Kit, (employed together with the Bioenno BDHC Substrate Kit), for dual-labeling immunohistochemistry.

Figure:  Bioenno DAB Substrate Kit employed together with the Bioenno BDHC Substrate Kit

(A,B) The Bioenno DAB Substrate Kit was used in standard avidin-biotin-peroxidase immunochemistry, which was performed on free-floating sections.  (C) Dual-labeling immunohistochemistry using the DAB Substrate Kit (brown color) and BDHC Substrate Kit (blue color).  DAB was used as the first chromogen and BDHC was the second one.  The boxed areas were magnified (63x) to highlight immuno-labeled neurons.  Images were taken from adult rat brains.

2017

• Running-induced memory enhancement correlates with the preservation of thin spines in the hippocampal area CA1 of old C57BL/6 mice 
  Benke Xu, Anbang Sun, Yun He, Feng Qian, Lian Liu, Yuncai Chen, Huanmin Luo(2017), Neurobiology of Aging,52, 106-116
  Link: http://www.sciencedirect.com/science/article/pii/S0197458017300027

2016

• Dual and opposing roles of microRNA-124 in epilepsy are mediated through inflammatory and NRSF-dependent gene networks
  Brennan GP, Dey D, Chen Y, Patterson KP, Magnetta EJ, Hall AM, Dube CM, Mei YT, Baram TZ (2016). Cell Reports, 14(10), 2402-2412.
  Link:  http://www.ncbi.nlm.nih.gov/pubmed/25134728